![]() Sample must be cleaned and free of interferents. Polymerase Chain Reaction Fluorescence īIORAD - QX200 Auto Droplet Generator.pdf|BIORAD - QX200 Droplet Reader.pdf|ĭroplet Generator: 11 x 14 x 5 in (28 x 36 x 13 cm), Droplet reader: 26 x 20 x 11 in (66 x 52 x 29 cm) The fraction of PCR-positive droplets enables the target to be quantified according to the Poisson distribution. Positive droplets, which contain at least one copy of the target DNR or RNA molecule, exhibit increased fluorescence compared to negative droplets. The droplets stream single file through the reader for fluorescence analysis. Droplets are transferred to a 96-well PCR plate and PCR is performed to end point in a thermal cycler. Each droplet serves to partition the reactions. ![]() ![]() The QX200 droplet generator, which can process eight samples at a time and requires samples to be manually pipetted into sample wells, partitions each sample into 20,000 uniform nanoliter-sized droplets in which nucleic acid molecules are randomly distributed. The Automated Droplet Generator simplifies the ddPCR workflow, making digital PCR both scalable and practical. The droplet generator is used with the QX200 droplet reader for EvaGreen or probe-based digital PCR applications. The QX200 AutoDG Droplet Digital PCR System provides absolute quantification of target DNA or RNA molecules for EvaGreen and probe-based Droplet Digital PCR (ddPCR) applications. The system consists of four instruments: a droplet generator, a plate sealer, a thermo cycler, and a droplet reader. Use the QX200 droplet generator to complete the first step of the Droplet Digital PCR (ddPCR) workflow by partitioning ddPCR reaction mix into 20,000 nanoliter-sized droplets for up to 8 samples per run. ![]() The Bio-Rad QX200 ddPCR System combines water-oil emulsion droplet technology with microfluidics. Automated Droplet Generator (Bio-Rad QX200 AutoDG ddPCR system) generates partitioned water-oil emulsion droplets (up to 20,000 droplets per 20ul reaction). ![]()
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